Abstract
Spirohexenolides A and B comprise a unique family of spirotetronate natural products. We report on the identification of their binding to and modulation of human macrophage migration inhibitor factor (hMIF). Using an immunoaffinity-fluorescent labeling method, the properties of this interaction are detailed and evidence is provided that hMIF plays a key role in the cytostatic activity of the spirohexenolides.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Heterocyclic Compounds, 4 or More Rings / chemistry
-
Heterocyclic Compounds, 4 or More Rings / pharmacology*
-
Humans
-
Intramolecular Oxidoreductases
-
Macrophage Migration-Inhibitory Factors / chemistry*
-
Macrophage Migration-Inhibitory Factors / metabolism
-
Macrophages / metabolism
Substances
-
Heterocyclic Compounds, 4 or More Rings
-
Macrophage Migration-Inhibitory Factors
-
spirohexenolide A
-
Intramolecular Oxidoreductases
-
phenylpyruvate tautomerase